NickelMaghrebV1, Main, Exploration, bibRecord, 000102

Production of Xylooligosaccharides by Immobilized His-tagged Recombinant Xylanase from Penicillium occitanis on Nickel-Chelate Eupergit C

Identifieur interne : 000102 ( Main/Exploration ); précédent : 000101; suivant : 000103

Production of Xylooligosaccharides by Immobilized His-tagged Recombinant Xylanase from Penicillium occitanis on Nickel-Chelate Eupergit C

Auteurs : Dorra Driss [Tunisie] ; Anissa Haddar [Tunisie] ; Raoudha Ghorbel [Tunisie] ; SEMIA ELLOUZ CHAABOUNI [Tunisie]

Source :

Applied biochemistry and biotechnology [ 0273-2289 ] ; 2014.

RBID : Pascal:14-0213286

Descripteurs français

Pascal (Inist)
- Production, Endo-1,4-β-xylanase, Xylan endo-1,3-β-xylosidase, Penicillium, Nickel, Immobilisation, Xylane.
Wicri :
- topic : Production, Nickel.

English descriptors

KwdEn :
- Endo-1,4-β-xylanase, Immobilization, Nickel, Penicillium, Production, Xylan, Xylan endo-1,3-β-xylosidase.

Abstract

Penicillium occitanis xylanase 2 expressed with a His-tag in Pichia pastoris, termed PoXyn2, was immobilized on nickel-chelate Eupergit C by covalent coupling reaction with a high immobilization yield up to 93.49 %. Characterization of the immobilized PoXyn2 was further evaluated. The optimum pH was not affected by immobilization, but the immobilized PoXyn2 exhibited more acidic and large optimum pH range (pH 2.0-4.0) than that of the free PoXyn2 (pH 3.0). The free PoXyn2 had an optimum temperature of 50 °C, whereas that of the immobilized enzyme was shifted to 65°C. Immobilization increased both pH stability and thermostability when compared with the free enzyme. Time courses of the xylooligosaccharides (XOS) produced from corncob xylan indicated that the immobilized enzyme tends to use shorter xylan chains and to produce more xylobiose and xylotriose initially. At the end of 24-h reaction, XOS mixture contained a total of 21.3 and 34.2 % (w/ w) of xylobiose and xylotriose with immobilized xylanase and free xylanase, respectively. The resulting XOS could be used as a special nutrient for lactic bacteria.

Affiliations:

Tunisie

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to stream PascalFrancis, to step Curation: 000425
to stream PascalFrancis, to step Checkpoint: 000016
to stream Main, to step Merge: 000103
to stream Main, to step Curation: 000102

Le document en format XML

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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Production of Xylooligosaccharides by Immobilized His-tagged Recombinant Xylanase from Penicillium occitanis on Nickel-Chelate Eupergit C</title>
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<series><title level="j" type="main">Applied biochemistry and biotechnology</title>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Endo-1,4-β-xylanase</term>
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<term>Nickel</term>
<term>Penicillium</term>
<term>Production</term>
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<front><div type="abstract" xml:lang="en">Penicillium occitanis xylanase 2 expressed with a His-tag in Pichia pastoris, termed PoXyn2, was immobilized on nickel-chelate Eupergit C by covalent coupling reaction with a high immobilization yield up to 93.49 %. Characterization of the immobilized PoXyn2 was further evaluated. The optimum pH was not affected by immobilization, but the immobilized PoXyn2 exhibited more acidic and large optimum pH range (pH 2.0-4.0) than that of the free PoXyn2 (pH 3.0). The free PoXyn2 had an optimum temperature of 50 °C, whereas that of the immobilized enzyme was shifted to 65°C. Immobilization increased both pH stability and thermostability when compared with the free enzyme. Time courses of the xylooligosaccharides (XOS) produced from corncob xylan indicated that the immobilized enzyme tends to use shorter xylan chains and to produce more xylobiose and xylotriose initially. At the end of 24-h reaction, XOS mixture contained a total of 21.3 and 34.2 % (w/ w) of xylobiose and xylotriose with immobilized xylanase and free xylanase, respectively. The resulting XOS could be used as a special nutrient for lactic bacteria.</div>
</front>
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<name sortKey="Semia Ellouz Chaabouni" sort="Semia Ellouz Chaabouni" uniqKey="Semia Ellouz Chaabouni" last="Semia Ellouz Chaabouni">SEMIA ELLOUZ CHAABOUNI</name>
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Production of Xylooligosaccharides by Immobilized His-tagged Recombinant Xylanase from Penicillium occitanis on Nickel-Chelate Eupergit C

Production of Xylooligosaccharides by Immobilized His-tagged Recombinant Xylanase from Penicillium occitanis on Nickel-Chelate Eupergit C

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri